Publications

Hautke A, Voronin A, Idiris F, Riel A, Lindner F, Lelièvre-Büttner A, Zhu J, Appel B, Fatti E, Weis K, Müller S, Schug A, Ebbinghaus S.
CAG-Repeat RNA Hairpin Folding and Recruitment to Nuclear Speckles with a Pivotal Role of ATP as a Cosolute
J Am Chem Soc. 2023 May 3;145(17):9571-9583. doi: 10.1021/jacs.2c13653.
external pagePMID: 37062072

Kralt A, Wojtynek M, Fischer JS, Agote-Aran A, Mancini R, Dultz E, Noor E, Uliana F, Tatarek-Nossol M, Antonin W, Onischenko E, Medalia O, Weis K.
An amphipathic helix in Brl1 is required for nuclear pore complex biogenesis in S. cerevisiae.
Elife. 2022 Aug 24;11:e78385. doi: 10.7554/eLife.78385.
external pagePMID: 36000978

Linsenmeier M, Hondele M, Grigolato F, Secchi E, Weis K, Arosio P.
Dynamic arrest and aging of biomolecular condensates are modulated by low-complexity domains, RNA and biochemical activity.
Nat Commun. 2022 May 31;13(1):3030. doi: 10.1038/s41467-022-30521-2.
external pagePMID: 35641495

Wollny D, Vernot B, Wang J, Hondele M, Safrastyan A, Aron F, Micheel J, He Z, Hyman A, Weis K, Camp JG, Tang TD, Treutlein B.
Characterization of RNA content in individual phase-separated coacervate microdroplets.
Nat Commun. 2022 May 12;13(1):2626. doi: 10.1038/s41467-022-30158-1.
external pagePMID: 35551426

Weis K, Hondele, M. The Role of DEAD-Box ATPases in Gene Expression and the Regulation of RNA-Protein Condensates.
Annu Rev Biochem 2022 Feb 18. doi: 10.1146/annurev-biochem-032620-105429.
external pagePMID: 35303788

Schuller AP, Wojtynek M, Mankus D, Tatli M, Kronenberg-Tenga R, Regmi SG, Dip PV, Lytton-Jean AKR, Brignole EJ, Dasso M, Weis K, Medalia O, Schwartz TU.
The cellular environment shapes the nuclear pore complex architecture.
Nature. 2021 Oct 13. doi: 10.1038/s41586-021-03985-3.
external pagePMID: 34646014

Weis K.
Dead or alive: DEAD-box ATPases as regulators of ribonucleoprotein complex condensation.
Biol Chem. 2021 Apr 2. doi: 10.1515/hsz-2020-0381.
external pagePMID: 33818025

Onischenko E, Noor E, Fischer JS, Gillet L, Wojtynek M, Vallotton P, Weis K.
Maturation Kinetics of a Multiprotein Complex Revealed by Metabolic Labeling.
Cell. 2020 Dec 11:S0092-8674(20)31460-4. doi: 10.1016/j.cell.2020.11.001.
external pagePMID: 33333025

Hondele M, Heinrich S, De Los Rios P, Weis K.
Membraneless organelles: phasing out of equilibrium.
Emerg Top Life Sci. 2020 Aug 3:ETLS20190190. doi: 10.1042/ETLS20190190.
external pagePMID: 32744309

Weber CA, Sekar K, Tang JH, Warmer P, Sauer U, Weis K.
β-Oxidation and autophagy are critical energy providers during acute glucose depletion in Saccharomyces cerevisiae.
Proc Natl Acad Sci U S A. 2020 May 19. pii: 201913370. doi: 10.1073/pnas.1913370117.
external pagePMID: 32430326

Kornmann B, Weis K.
Liquid but not contactless.
Science. 2020 Jan 31;367(6477):507-508. doi: 10.1126/science.aba3771.
external pagePMID: 32001641

Derrer CP, Mancini R, Vallotton P, Huet S, Weis K, Dultz E.
The RNA export factor Mex67 functions as a mobile nucleoporin.
J Cell Biol. 2019 Nov 21. pii: jcb.201909028. doi: 10.1083/jcb.201909028.
external pagePMID: 31753862

Hondele M, Sachdev R, Heinrich S, Wang J, Vallotton P, Fontoura BMA, Weis K.
DEAD-box ATPases are global regulators of phase-separated organelles.
Nature. 2019 Aug 21. doi: 10.1038/s41586-019-1502-y.
external pagePMID: 31435012

Linsenmeier M, Kopp MRG, Grigolato F, Emmanouilidis L, Liu D, Zürcher D, Hondele M, Weis K, Palmiero UC, Arosio P.
Dynamics of synthetic membraneless organelles in microfluidic droplets.
Angew Chem Int Ed Engl. 2019 Jul 23. doi: 10.1002/anie.201907278.
external pagePMID: 31334587

Vallotton P, Rajoo S, Wojtynek M, Onischenko E, Kralt A, Derrer CP,
Weis K.
Mapping the native organization of the yeast nuclear pore complex using nuclear radial intensity measurements.
Proc Natl Acad Sci U S A. 2019 Jul 1. pii: 201903764. doi: 10.1073/pnas.1903764116.
external pagePMID: 31262825

Sachdev R, Hondele M, Linsenmeier M, Vallotton P, Mugler CF, Arosio P, Weis K.
Pat1 promotes processing body assembly by enhancing the phase separation of the DEAD-box ATPase Dhh1 and RNA.
Elife. 2019 Jan 16;8. pii: e41415. doi: 10.7554/eLife.41415.
external pagePMID: 30648970

Cheng Z, Mugler CF, Keskin A, Hodapp S, Chan LY, Weis K, Mertins P, Regev A, Jovanovic M, Brar GA.
Small and Large Ribosomal Subunit Deficiencies Lead to Distinct Gene Expression Signatures that Reflect Cellular Growth Rate.
Mol Cell. 2018 Nov 27. pii: S1097-2765(18)30892-X. doi: 10.1016/j.molcel.2018.10.032.
external pagePMID: 30503772

Faltova L, Küffner AM, Hondele M, Weis K, Arosio P.
Multifunctional Protein Materials and Microreactors using Low Complexity Domains as Molecular Adhesives.
ACS Nano. 2018 Sep 14. doi: 10.1021/acsnano.8b04304.
external pagePMID: 30216718

Chan LY, Mugler CF, Heinrich S, Vallotton P, Weis K.
Non-invasive measurement of mRNA decay reveals translation initiation as the major determinant of mRNA stability.
Elife. 2018 Sep 7;7. pii: e32536. doi: 10.7554/eLife.32536.
external pagePMID: 30192227

Dultz E, Mancini R, Polles G, Vallotton P, Alber F, Weis K.
Quantitative imaging of chromatin decompaction in living cells.
Mol Biol Cell. 2018 May 17:mbcE17110648. doi: 10.1091/mbc.E17-11-0648.
external pagePMID: 29771637

Rajoo S*, Vallotton P*, Onischenko E, Weis K. Stoichiometry and compositional plasticity of the yeast nuclear pore complex revealed by quantitative fluorescence microscopy.
Proc Natl Acad Sci U S A. 2018 Apr 9. pii: 201719398. doi: 10.1073/pnas.1719398115.
external pagePMID: 29632211

Shechtman Y, Gustavsson AK, Petrov PN, Dultz E, Lee MY, Weis K,
Moerner WE. Observation of live chromatin dynamics in cells via 3D localization microscopy using Tetrapod point spread functions.
Biomed Opt Express. 2017 Nov 21;8(12):5735-5748. doi: 10.1364/BOE.8.005735. eCollection 2017 Dec 1.
external pagePMID: 29296501

Onischenko E, Tang JH, Andersen KR, Knockenhauer KE, Vallotton P, Derrer CP, Kralt A, Mugler CF, Chan LY, Schwartz TU, Weis K. Natively Unfoldes FG Repeats Stabilize the Structure of the Nuclear Pore Complex.
Cell. 2017 Oct 7. pii: S0092-8674(17)31127-3. doi: 10.1016/j.cell.2017.09.033.
external pagePMID: 29033133

Vallotton P, van Oijen AM, Whitchurch CB, Gelfand V, Yeo L, Tsiavaliaris G, Heinrich S, Dultz E, Weis K, Grünwald D. Diatrack particle tracking software: review of applications and performance evaluation.
Traffic. 2017 Sep 25. doi: 10.1111/tra.12530.
external pagePMID: 28945316

Heinrich S, Sidler CL, Azzalin CM, Weis K. Stem-loop RNA labeling can affect nuclear and cytoplasmic mRNA processing.
RNA. 2017 Feb; 23(2):134-141. doi: 10.1261/rna.057786.116.
external pagePMID: 28096443

Heinrich S, Derrer CP, Lari A, Weis K, Montpetit B. Temporal and spatial regulation of mRNA export: Single particle RNA-imaging provides new tools and insights.
Bioessays. 2017 Jan 4. doi: 10.1002/bies.201600124.
external pagePMID: 28052353

Mugler CF*, Hondele M*, Heinrich S, Sachdev R, Vallotton P, Koek AY, Chan LY, Weis K. ATPase activity of the DEAD-box protein Dhh1 controls processing body formation.
eLife. 2016 Oct 3;5. pii: e18746. doi: 10.7554/eLife 18746.
external pagePMID: 27692063

Joyner RP, Tang JH, Helenius J, Dultz E, Brune C, Holt LJ, Huet S, Müller DJ, & Weis K. (2016) A glucose-starvation response regulates the diffusion of macromolecules.
eLife 2016 Mar 22;5:e09376.
external pagePMID: 27003290

Dultz E, Tjong H, Weider E, Herzog M, Young B, Brune C, Müllner D, Loewen C, Alber F, & Weis K. (2016) Global reorganization of budding yeast chromosome conformation in different physiological conditions.
J Cell Biol 2016 Feb 1;212(3):321-34.
external pagePMID: 26811423

Smith C*, Lari A*, Derrer CP*, Ouwehand A, Rossouw A, Huisman M, Dange T, Hopman M, Joseph A, Zenklusen D, Weis K, Grunwald D, & Montpetit B. (2015) In vivo single-particle imaging of nuclear mRNA export in budding yeast demonstrates an essential role for Mex67p.
J Cell Biol 2015 Dec 21;211(6):1121-30.
external pagePMID:26694837

Lowe AR*, Tang JH*, Yassif J, Graf M, Huang WY, Groves JT, Weis K, & Liphardt JT. (2015)
Importin-β modulates the permeability of the nuclear pore complex in a Ran-dependent manner.
eLife 2015 Mar 6;4:e04052.
external pagePMID: 25748139

Backlund MP, Joyner R, Weis K, & Moerner WE. (2014)
Correlations of three-dimensional motion of chromosomal loci in yeast revealed by the double-helix point spread function microscope.
Mol Biol Cell 2014 Nov 5;25(22):3619-29. doi: 10.1091/mbc.E14-06-1127. Epub 2014 Oct 15.
external pagePMID: 25318676

Azimi M, Bulat E, Weis K, & Mofrad MR. (2014)
An agent-based model for mRNA export through the nuclear pore complex.
Mol Biol Cell 2014 Nov 5;25(22):3643-53. doi: 10.1091/mbc.E14-06-1065. Epub 2014 Sep 24.
external pagePMID: 25253717

Andersen KR*, Onischenko E*, Tang JH, Kumar P, Chen JZ, Ulrich A, Liphardt JT, Weis K, & Schwartz TU. (2013)
Scaffold nucleoporins Nup188 and Nup192 share structural and functional properties with nuclear transport receptors.
eLife 2013 Jun 11;2:e00745.
external pagePMID: 23795296

Bird SL, Heald R, & Weis K. (2013)
RanGTP and CLASP1 cooperate to position the mitotic spindle.
Mol Biol Cell 24:2506-14.
external pagePMID: 23783028

Montpetit B, Seeliger MA, & Weis K. (2012)
Analysis of DEAD-box proteins in mRNA export.
Methods Enzymol 511:239-54.
external pagePMID: 22713323

Montpetit B & Weis K. (2012)
An alternative route for nuclear mRNP export by membrane budding.
Science 336:809-10.
external pagePMID: 22605737

Green EM*, Jiang Y*, Joyner R, & Weis K. (2012)
A negative feedback loop at the nuclear periphery regulates GAL gene expression.
Mol Biol Cell 23:1367-75.
external pagePMID: 22323286

Halpin D, Kalab P, Wang J, Weis K, & Heald R. (2011)
Mitotic spindle assembly around RCC1-coated beads in Xenopus egg extracts.
PLoS Biol 9(12):e1001225. Epub 2011 Dec 27.
external pagePMID: 22215983

Carroll JS, Munchel SE, & Weis K. (2011)
The DExD/H box ATPase Dhh1 functions in translational repression, mRNA decay and P body dynamics.
J Cell Biol 194:527-37.
external pagePMID: 21844211

Munchel SE, Shultzaberger RK, Takizawa N, & Weis K. (2011)
Dynamic profiling of mRNA turnover reveals gene-specific and system-wide regulation of mRNA decay.
Mol Biol Cell 22:2787-95.
external pagePMID: 21680716

Montpetit B*, Thomsen ND*, Helmke KJ, Seeliger MA, Berger JM, & Weis K. (2011)
A conserved mechanism of DEAD-box ATPase activation by nucleoporins and IP₆ in RNA Export.
Nature 472:238-42.
external pagePMID: 21441902

Soderholm JF, Bird SL, Kalab P, Sampathkumar Y, Hasegawa K, Uehara-Bingen M, Weis K, & Heald R. (2011)
Importazole, a small molecule inhibitor of the transport receptor importin-β.
ACS Chem Biol 6(7):700-8.
external pagePMID: 21469738

Onischenko E & Weis K. (2011)
Nuclear pore complex - a coat specifically tailored for the nuclear envelope.
Curr. Opin. Cell Bio, Feb 4.

Lowe AR*, Siegel JJ*, Kalab P, Siu M, Weis K, & Liphardt JT. (2010)
Selectivity mechanism of the nuclear pore complex characterized by single cargo tracking.
Nature 467:600-3.

Dossani ZY, Weirich CS, Erzberger JP, Berger JM, & Weis K. (2009)
Structure of the C-terminus of the mRNA export factor Dbp5 reveals the interaction surface for the ATPase activator Gle1.
Proc Natl Acad Sci U S A 106:16251-6.

Onischenko E, Stanton LH, Madrid AS, Kieselbach T, & Weis K. (2009)
Role of the Ndc1-interaction network in yeast nuclear pore complex assembly and maintenance.
JCB 185, 475-91.

MakioT, Stanton LH, Lin C, Goldfarb DS, Weis K, & Wozniak RW. (2009)
The related nucleoporins Nup170p and Nup157p share an essential function in nuclear pore complex biogenesis.
JCB 185, 459-73.

Abu-Arish A, Kalab P, Ng-Kamstra J, Weis K, & Fradin C. (2009)
Spatial distribution and mobility of the Ran GTPase in live interphase cells.
Biophys J 97:2164-78.

Blower MD, Weis K, & Heald R. (2007)
Genome-wide analysis demonstrates the conserved localization of specific mRNAs to mitotic microtubules.
JCB 179, 1365-73.

Weis K. (2007)
The nuclear pore complex: oily spaghetti or gummy bear?
Cell 130, 405-7.

Weirich CS, Erzberger JP, Flick JS, Berger JM, Thorner J, & Weis K. (2006)
Local activation of the DExD/H-box protein Dbp5 by the nuclear pore protein Gle1 and its coactivator inositol hexakisphosphate is required for mRNA export.
Nature Cell Biol 8, 668-76.

Madrid AS, Mancuso J, Cande WZ, & Weis K. (2006)
The role of the integral membrane nucleoporins Ndc1p and Pom152p in nuclear pore complex assembly and function.
JCB 173, 361-71.

Kalab P*, Pralle A*, Isacoff EY, Heald R, & Weis K. (2006)
Analysis of a RanGTP-regulated gradient in somatic cells.
Nature 440:697-701.

Soderholm J, Uehara-Bingen M, Weis K, & Heald R. (2006)
Challenges facing the biologist doing chemical genetics.
Nat Chem Biol 2:55-58

Madrid AS & Weis K. (2006)
Nuclear transport is becoming crystal clear.
Chromosoma 115:98-109.

Maresca TJ, Niederstrasser H, Weis K, & Heald R. (2005)
Xnf7 contributes to spindle integrity through its microtubule bundling activity.
Curr Biol 15:1755-61.

Santama N, Ogg SC, Malekkou A, Zographos SE, Weis K, & Lamond AI. (2005)
Characterization of hCINAP, a novel coilin-interacting protein encoded by a transcript from the transcription factor TAFIID32 locus.
JBC 280:36429-41.

Blower MD*, Nachury MV*, Heald R, & Weis K. (2005)
A Rae1-containing ribonucleoprotein complex is required for mitotic spindle assembly.
Cell 121: 223-34.

Brune C, Munchel SE, Fischer N, Potdtelejnikov AV, & Weis K. (2005)
Yeast poly (A)-binding protein Pab1 shuttles between the cytoplasm and the nucleus and functions in mRNA export.
RNA 4, 517-31.

Weirich CS, Erzberger JP, Berger JM, & Weis K. (2004)
The N-terminal domain of Nup159 forms a b-propeller that functions in mRNA export by tethering the helicase Dbp5 to the nuclear pore.
Molecular Cell 16, 749-60.

Zeitler B & Weis K. (2004)
The asymmetry of FG-repeat nucleoporins is dispensable for bulk transport through the nuclear pore complex in vivo.
JCB 167: 583-590

Weis K. (2003)
Regulating access to the genome: nucleocytoplasmic transport throughout the cell cycle.
Cell 112, 441-451

Kalab P, Weis K, & Heald R. (2002)
Visualization of a RanGTP gradient in interphase and mitotic Xenopus egg extracts.
Science 295, 2452-2456.

Nachury MV, Maresca TJ, Salmon WC, Waterman-Storer CM, Heald R, & Weis K. (2001)
Importin-β is a mitotic target of the small GTPase Ran in spindle assembly.
Cell 104, 95–106.

Nachury MV & Weis K. (1999)
The direction of transport through the nuclear pore can be inverted.
Proc Natl Acad Sci USA 96, 9622-27.

Stade K, Ford CF, Guthrie C, & Weis K. (1997)
Exportin 1 (Crm1p) is an essential nuclear export factor.
Cell 90, 1041-1050.

Weis K, Mattaj IW, & Lamond AI. (1995)
Identification of hSRP1a as a functional receptor for nuclear localization sequences.
Science 268, 1049-53.

Weis K, Rambaud S, Carvalho T, Carmo-Fonseca M, Lavau C, Jansen J, Lamond AI, & Dejean A. (1994)
Retinoic acid regulates aberrant nuclear localization of PML-RARa in acute promyelocytic leukaemia cells.
Cell 76, 345-56.

* denotes equal contribution;

Publications

Links