Publications
Heinrich S, Hondele M, Marchand D, Derrer CP, Zedan M, Oswald A, Malinovska L, Uliana F, Khawaja S, Mancini R, Grunwald D, Weis K.
Glucose stress causes mRNA retention in nuclear Nab2 condensates.
Cell Rep. 2024 Jan 23;43(1):113593. doi: 10.1016/j.celrep.2023.113593.
external page PMID: 38113140
Hautke A, Voronin A, Idiris F, Riel A, Lindner F, Lelièvre-Büttner A, Zhu J, Appel B, Fatti E, Weis K, Müller S, Schug A, Ebbinghaus S.
CAG-Repeat RNA Hairpin Folding and Recruitment to Nuclear Speckles with a Pivotal Role of ATP as a Cosolute
J Am Chem Soc. 2023 May 3;145(17):9571-9583. doi: 10.1021/jacs.2c13653.
external page PMID: 37062072
Kralt A, Wojtynek M, Fischer JS, Agote-Aran A, Mancini R, Dultz E, Noor E, Uliana F, Tatarek-Nossol M, Antonin W, Onischenko E, Medalia O, Weis K.
An amphipathic helix in Brl1 is required for nuclear pore complex biogenesis in S. cerevisiae.
Elife. 2022 Aug 24;11:e78385. doi: 10.7554/eLife.78385.
external page PMID: 36000978
Linsenmeier M, Hondele M, Grigolato F, Secchi E, Weis K, Arosio P.
Dynamic arrest and aging of biomolecular condensates are modulated by low-complexity domains, RNA and biochemical activity.
Nat Commun. 2022 May 31;13(1):3030. doi: 10.1038/s41467-022-30521-2.
external page PMID: 35641495
Wollny D, Vernot B, Wang J, Hondele M, Safrastyan A, Aron F, Micheel J, He Z, Hyman A, Weis K, Camp JG, Tang TD, Treutlein B.
Characterization of RNA content in individual phase-separated coacervate microdroplets.
Nat Commun. 2022 May 12;13(1):2626. doi: 10.1038/s41467-022-30158-1.
external page PMID: 35551426
Weis K, Hondele, M. The Role of DEAD-Box ATPases in Gene Expression and the Regulation of RNA-Protein Condensates.
Annu Rev Biochem 2022 Feb 18. doi: 10.1146/annurev-biochem-032620-105429.
external page PMID: 35303788
Schuller AP, Wojtynek M, Mankus D, Tatli M, Kronenberg-Tenga R, Regmi SG, Dip PV, Lytton-Jean AKR, Brignole EJ, Dasso M, Weis K, Medalia O, Schwartz TU.
The cellular environment shapes the nuclear pore complex architecture.
Nature. 2021 Oct 13. doi: 10.1038/s41586-021-03985-3.
external page PMID: 34646014
Weis K.
Dead or alive: DEAD-box ATPases as regulators of ribonucleoprotein complex condensation.
Biol Chem. 2021 Apr 2. doi: 10.1515/hsz-2020-0381.
external page PMID: 33818025
Onischenko E, Noor E, Fischer JS, Gillet L, Wojtynek M, Vallotton P, Weis K.
Maturation Kinetics of a Multiprotein Complex Revealed by Metabolic Labeling.
Cell. 2020 Dec 11:S0092-8674(20)31460-4. doi: 10.1016/j.cell.2020.11.001.
external page PMID: 33333025
Hondele M, Heinrich S, De Los Rios P, Weis K.
Membraneless organelles: phasing out of equilibrium.
Emerg Top Life Sci. 2020 Aug 3:ETLS20190190. doi: 10.1042/ETLS20190190.
external page PMID: 32744309
Weber CA, Sekar K, Tang JH, Warmer P, Sauer U, Weis K.
β-Oxidation and autophagy are critical energy providers during acute glucose depletion in Saccharomyces cerevisiae.
Proc Natl Acad Sci U S A. 2020 May 19. pii: 201913370. doi: 10.1073/pnas.1913370117.
external page PMID: 32430326
Kornmann B, Weis K.
Liquid but not contactless.
Science. 2020 Jan 31;367(6477):507-508. doi: 10.1126/science.aba3771.
external page PMID: 32001641
Derrer CP, Mancini R, Vallotton P, Huet S, Weis K, Dultz E.
The RNA export factor Mex67 functions as a mobile nucleoporin.
J Cell Biol. 2019 Nov 21. pii: jcb.201909028. doi: 10.1083/jcb.201909028.
external page PMID: 31753862
Hondele M, Sachdev R, Heinrich S, Wang J, Vallotton P, Fontoura BMA, Weis K.
DEAD-box ATPases are global regulators of phase-separated organelles.
Nature. 2019 Aug 21. doi: 10.1038/s41586-019-1502-y.
external page PMID: 31435012
Linsenmeier M, Kopp MRG, Grigolato F, Emmanouilidis L, Liu D, Zürcher D, Hondele M, Weis K, Palmiero UC, Arosio P.
Dynamics of synthetic membraneless organelles in microfluidic droplets.
Angew Chem Int Ed Engl. 2019 Jul 23. doi: 10.1002/anie.201907278.
external page PMID: 31334587
Vallotton P, Rajoo S, Wojtynek M, Onischenko E, Kralt A, Derrer CP,
Weis K.
Mapping the native organization of the yeast nuclear pore complex using nuclear radial intensity measurements.
Proc Natl Acad Sci U S A. 2019 Jul 1. pii: 201903764. doi: 10.1073/pnas.1903764116.
external page PMID: 31262825
Sachdev R, Hondele M, Linsenmeier M, Vallotton P, Mugler CF, Arosio P, Weis K.
Pat1 promotes processing body assembly by enhancing the phase separation of the DEAD-box ATPase Dhh1 and RNA.
Elife. 2019 Jan 16;8. pii: e41415. doi: 10.7554/eLife.41415.
external page PMID: 30648970
Cheng Z, Mugler CF, Keskin A, Hodapp S, Chan LY, Weis K, Mertins P, Regev A, Jovanovic M, Brar GA.
Small and Large Ribosomal Subunit Deficiencies Lead to Distinct Gene Expression Signatures that Reflect Cellular Growth Rate.
Mol Cell. 2018 Nov 27. pii: S1097-2765(18)30892-X. doi: 10.1016/j.molcel.2018.10.032.
external page PMID: 30503772
Faltova L, Küffner AM, Hondele M, Weis K, Arosio P.
Multifunctional Protein Materials and Microreactors using Low Complexity Domains as Molecular Adhesives.
ACS Nano. 2018 Sep 14. doi: 10.1021/acsnano.8b04304.
external page PMID: 30216718
Chan LY, Mugler CF, Heinrich S, Vallotton P, Weis K.
Non-invasive measurement of mRNA decay reveals translation initiation as the major determinant of mRNA stability.
Elife. 2018 Sep 7;7. pii: e32536. doi: 10.7554/eLife.32536.
external page PMID: 30192227
Dultz E, Mancini R, Polles G, Vallotton P, Alber F, Weis K.
Quantitative imaging of chromatin decompaction in living cells.
Mol Biol Cell. 2018 May 17:mbcE17110648. doi: 10.1091/mbc.E17-11-0648.
external page PMID: 29771637
Rajoo S*, Vallotton P*, Onischenko E, Weis K. Stoichiometry and compositional plasticity of the yeast nuclear pore complex revealed by quantitative fluorescence microscopy.
Proc Natl Acad Sci U S A. 2018 Apr 9. pii: 201719398. doi: 10.1073/pnas.1719398115.
external page PMID: 29632211
Shechtman Y, Gustavsson AK, Petrov PN, Dultz E, Lee MY, Weis K,
Moerner WE. Observation of live chromatin dynamics in cells via 3D localization microscopy using Tetrapod point spread functions.
Biomed Opt Express. 2017 Nov 21;8(12):5735-5748. doi: 10.1364/BOE.8.005735. eCollection 2017 Dec 1.
external page PMID: 29296501
Onischenko E, Tang JH, Andersen KR, Knockenhauer KE, Vallotton P, Derrer CP, Kralt A, Mugler CF, Chan LY, Schwartz TU, Weis K. Natively Unfoldes FG Repeats Stabilize the Structure of the Nuclear Pore Complex.
Cell. 2017 Oct 7. pii: S0092-8674(17)31127-3. doi: 10.1016/j.cell.2017.09.033.
external page PMID: 29033133
Vallotton P, van Oijen AM, Whitchurch CB, Gelfand V, Yeo L, Tsiavaliaris G, Heinrich S, Dultz E, Weis K, Grünwald D. Diatrack particle tracking software: review of applications and performance evaluation.
Traffic. 2017 Sep 25. doi: 10.1111/tra.12530.
external page PMID: 28945316
Heinrich S, Sidler CL, Azzalin CM, Weis K. Stem-loop RNA labeling can affect nuclear and cytoplasmic mRNA processing.
RNA. 2017 Feb; 23(2):134-141. doi: 10.1261/rna.057786.116.
external page PMID: 28096443
Heinrich S, Derrer CP, Lari A, Weis K, Montpetit B. Temporal and spatial regulation of mRNA export: Single particle RNA-imaging provides new tools and insights.
Bioessays. 2017 Jan 4. doi: 10.1002/bies.201600124.
external page PMID: 28052353
Mugler CF*, Hondele M*, Heinrich S, Sachdev R, Vallotton P, Koek AY, Chan LY, Weis K. ATPase activity of the DEAD-box protein Dhh1 controls processing body formation.
eLife. 2016 Oct 3;5. pii: e18746. doi: 10.7554/eLife 18746.
external page PMID: 27692063
Joyner RP, Tang JH, Helenius J, Dultz E, Brune C, Holt LJ, Huet S, Müller DJ, & Weis K. (2016) A glucose-starvation response regulates the diffusion of macromolecules.
eLife 2016 Mar 22;5:e09376.
external page PMID: 27003290
Dultz E, Tjong H, Weider E, Herzog M, Young B, Brune C, Müllner D, Loewen C, Alber F, & Weis K. (2016) Global reorganization of budding yeast chromosome conformation in different physiological conditions.
J Cell Biol 2016 Feb 1;212(3):321-34.
external page PMID: 26811423
Smith C*, Lari A*, Derrer CP*, Ouwehand A, Rossouw A, Huisman M, Dange T, Hopman M, Joseph A, Zenklusen D, Weis K, Grunwald D, & Montpetit B. (2015) In vivo single-particle imaging of nuclear mRNA export in budding yeast demonstrates an essential role for Mex67p.
J Cell Biol 2015 Dec 21;211(6):1121-30.
external page PMID:26694837
Lowe AR*, Tang JH*, Yassif J, Graf M, Huang WY, Groves JT, Weis K, & Liphardt JT. (2015)
Importin-β modulates the permeability of the nuclear pore complex in a Ran-dependent manner.
eLife 2015 Mar 6;4:e04052.
external page PMID: 25748139
Backlund MP, Joyner R, Weis K, & Moerner WE. (2014)
Correlations of three-dimensional motion of chromosomal loci in yeast revealed by the double-helix point spread function microscope.
Mol Biol Cell 2014 Nov 5;25(22):3619-29. doi: 10.1091/mbc.E14-06-1127. Epub 2014 Oct 15.
external page PMID: 25318676
Azimi M, Bulat E, Weis K, & Mofrad MR. (2014)
An agent-based model for mRNA export through the nuclear pore complex.
Mol Biol Cell 2014 Nov 5;25(22):3643-53. doi: 10.1091/mbc.E14-06-1065. Epub 2014 Sep 24.
external page PMID: 25253717
Andersen KR*, Onischenko E*, Tang JH, Kumar P, Chen JZ, Ulrich A, Liphardt JT, Weis K, & Schwartz TU. (2013)
Scaffold nucleoporins Nup188 and Nup192 share structural and functional properties with nuclear transport receptors.
eLife 2013 Jun 11;2:e00745.
external page PMID: 23795296
Bird SL, Heald R, & Weis K. (2013)
RanGTP and CLASP1 cooperate to position the mitotic spindle.
Mol Biol Cell 24:2506-14.
external page PMID: 23783028
Montpetit B, Seeliger MA, & Weis K. (2012)
Analysis of DEAD-box proteins in mRNA export.
Methods Enzymol 511:239-54.
external page PMID: 22713323
Montpetit B & Weis K. (2012)
An alternative route for nuclear mRNP export by membrane budding.
Science 336:809-10.
external page PMID: 22605737
Green EM*, Jiang Y*, Joyner R, & Weis K. (2012)
A negative feedback loop at the nuclear periphery regulates GAL gene expression.
Mol Biol Cell 23:1367-75.
external page PMID: 22323286
Halpin D, Kalab P, Wang J, Weis K, & Heald R. (2011)
Mitotic spindle assembly around RCC1-coated beads in Xenopus egg extracts.
PLoS Biol 9(12):e1001225. Epub 2011 Dec 27.
external page PMID: 22215983
Carroll JS, Munchel SE, & Weis K. (2011)
The DExD/H box ATPase Dhh1 functions in translational repression, mRNA decay and P body dynamics.
J Cell Biol 194:527-37.
external page PMID: 21844211
Munchel SE, Shultzaberger RK, Takizawa N, & Weis K. (2011)
Dynamic profiling of mRNA turnover reveals gene-specific and system-wide regulation of mRNA decay.
Mol Biol Cell 22:2787-95.
external page PMID: 21680716
Montpetit B*, Thomsen ND*, Helmke KJ, Seeliger MA, Berger JM, & Weis K. (2011)
A conserved mechanism of DEAD-box ATPase activation by nucleoporins and IP6 in RNA Export.
Nature 472:238-42.
external page PMID: 21441902
Soderholm JF, Bird SL, Kalab P, Sampathkumar Y, Hasegawa K, Uehara-Bingen M, Weis K, & Heald R. (2011)
Importazole, a small molecule inhibitor of the transport receptor importin-β.
ACS Chem Biol 6(7):700-8.
external page PMID: 21469738
Onischenko E & Weis K. (2011)
Nuclear pore complex - a coat specifically tailored for the nuclear envelope.
Curr. Opin. Cell Bio, Feb 4.
Lowe AR*, Siegel JJ*, Kalab P, Siu M, Weis K, & Liphardt JT. (2010)
Selectivity mechanism of the nuclear pore complex characterized by single cargo tracking.
Nature 467:600-3.
Dossani ZY, Weirich CS, Erzberger JP, Berger JM, & Weis K. (2009)
Structure of the C-terminus of the mRNA export factor Dbp5 reveals the interaction surface for the ATPase activator Gle1.
Proc Natl Acad Sci U S A 106:16251-6.
Onischenko E, Stanton LH, Madrid AS, Kieselbach T, & Weis K. (2009)
Role of the Ndc1-interaction network in yeast nuclear pore complex assembly and maintenance.
JCB 185, 475-91.
Makio T, Stanton LH, Lin C, Goldfarb DS, Weis K, & Wozniak RW. (2009)
The related nucleoporins Nup170p and Nup157p share an essential function in nuclear pore complex biogenesis.
JCB 185, 459-73.
Abu-Arish A, Kalab P, Ng-Kamstra J, Weis K, & Fradin C. (2009)
Spatial distribution and mobility of the Ran GTPase in live interphase cells.
Biophys J 97:2164-78.
Blower MD, Weis K, & Heald R. (2007)
Genome-wide analysis demonstrates the conserved localization of specific mRNAs to mitotic microtubules.
JCB 179, 1365-73.
Weis K. (2007)
The nuclear pore complex: oily spaghetti or gummy bear?
Cell 130, 405-7.
Weirich CS, Erzberger JP, Flick JS, Berger JM, Thorner J, & Weis K. (2006)
Local activation of the DExD/H-box protein Dbp5 by the nuclear pore protein Gle1 and its coactivator inositol hexakisphosphate is required for mRNA export.
Nature Cell Biol 8, 668-76.
Madrid AS, Mancuso J, Cande WZ, & Weis K. (2006)
The role of the integral membrane nucleoporins Ndc1p and Pom152p in nuclear pore complex assembly and function.
JCB 173, 361-71.
Kalab P*, Pralle A*, Isacoff EY, Heald R, & Weis K. (2006)
Analysis of a RanGTP-regulated gradient in somatic cells.
Nature 440:697-701.
Soderholm J, Uehara-Bingen M, Weis K, & Heald R. (2006)
Challenges facing the biologist doing chemical genetics.
Nat Chem Biol 2:55-58
Madrid AS & Weis K. (2006)
Nuclear transport is becoming crystal clear.
Chromosoma 115:98-109.
Maresca TJ, Niederstrasser H, Weis K, & Heald R. (2005)
Xnf7 contributes to spindle integrity through its microtubule bundling activity.
Curr Biol 15:1755-61.
Santama N, Ogg SC, Malekkou A, Zographos SE, Weis K, & Lamond AI. (2005)
Characterization of hCINAP, a novel coilin-interacting protein encoded by a transcript from the transcription factor TAFIID32 locus.
JBC 280:36429-41.
Blower MD*, Nachury MV*, Heald R, & Weis K. (2005)
A Rae1-containing ribonucleoprotein complex is required for mitotic spindle assembly.
Cell 121: 223-34.
Brune C, Munchel SE, Fischer N, Potdtelejnikov AV, & Weis K. (2005)
Yeast poly (A)-binding protein Pab1 shuttles between the cytoplasm and the nucleus and functions in mRNA export.
RNA 4, 517-31.
Weirich CS, Erzberger JP, Berger JM, & Weis K. (2004)
The N-terminal domain of Nup159 forms a b-propeller that functions in mRNA export by tethering the helicase Dbp5 to the nuclear pore.
Molecular Cell 16, 749-60.
Zeitler B & Weis K. (2004)
The asymmetry of FG-repeat nucleoporins is dispensable for bulk transport through the nuclear pore complex in vivo.
JCB 167: 583-590
Weis K. (2003)
Regulating access to the genome: nucleocytoplasmic transport throughout the cell cycle.
Cell 112, 441-451
Kalab P, Weis K, & Heald R. (2002)
Visualization of a RanGTP gradient in interphase and mitotic Xenopus egg extracts.
Science 295, 2452-2456.
Nachury MV, Maresca TJ, Salmon WC, Waterman-Storer CM, Heald R, & Weis K. (2001)
Importin-β is a mitotic target of the small GTPase Ran in spindle assembly.
Cell 104, 95–106.
Nachury MV & Weis K. (1999)
The direction of transport through the nuclear pore can be inverted.
Proc Natl Acad Sci USA 96, 9622-27.
Stade K, Ford CF, Guthrie C, & Weis K. (1997)
Exportin 1 (Crm1p) is an essential nuclear export factor.
Cell 90, 1041-1050.
Weis K, Mattaj IW, & Lamond AI. (1995)
Identification of hSRP1a as a functional receptor for nuclear localization sequences.
Science 268, 1049-53.
Weis K, Rambaud S, Carvalho T, Carmo-Fonseca M, Lavau C, Jansen J, Lamond AI, & Dejean A. (1994)
Retinoic acid regulates aberrant nuclear localization of PML-RARa in acute promyelocytic leukaemia cells.
Cell 76, 345-56.
* denotes equal contribution;